Hotspot

trnE-UUC-trnT-GGU

LSC · 33256-33967 · 712 bp

Back to Microtropis

Pi 0.00467

MarkerSeek score 64.90

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 3
pi: 0.00467
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 64.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CCTAACCCTACTACCCCCAG	CCATGGCGTTACTCTACCAC	60.0	55.0	57.9	58.1	761-830 bp	1.0	83.0
trnE-UUC-trnT-GGU_p2	CTAACCCTACTACCCCCAGG	CCATGGCGTTACTCTACCAC	60.0	55.0	57.9	58.1	760-829 bp	1.0	83.0
trnE-UUC-trnT-GGU_p3	CCTAACCCTACTACCCCCAG	ATATGAATACTCCCGCGGGA	60.0	50.0	57.9	58.1	833-902 bp	1.0	83.0
trnE-UUC-trnT-GGU_p4	CTAACCCTACTACCCCCAGG	ATATGAATACTCCCGCGGGA	60.0	50.0	57.9	58.1	832-901 bp	1.0	83.0
trnE-UUC-trnT-GGU_p5	CTAACCCTACTACCCCCAGG	TGGCGTTACTCTACCACTGA	60.0	50.0	57.9	58.1	757-826 bp	1.0	82.8