Hotspot

petN-psbM

LSC · 30085-31247 · 1163 bp

Back to Microtropis

Pi 0.00618

MarkerSeek score 64.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1163
variable_sites: 7
pi: 0.00618
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CACTCGTAGTATGGGGACGA	AGTTCCTACTGCCTTTTTGCT	55.0	42.9	58.3	57.8	1239-1268 bp	1.0	69.9
petN-psbM_p2	TCACTCGTAGTATGGGGACG	AGTTCCTACTGCCTTTTTGCT	55.0	42.9	58.3	57.8	1240-1269 bp	1.0	69.9
petN-psbM_p3	ACTCGTAGTATGGGGACGAA	AGTTCCTACTGCCTTTTTGCT	50.0	42.9	57.6	57.8	1238-1267 bp	1.0	69.1
petN-psbM_p4	CACTCGTAGTATGGGGACGA	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.3	57.6	1260-1289 bp	1.0	68.4
petN-psbM_p5	TCACTCGTAGTATGGGGACG	TGCTACTGCACTGTTCATTCT	55.0	42.9	58.3	57.6	1261-1290 bp	1.0	68.4