Hotspot

rpoB-trnC-GCA

LSC · 27887-29005 · 1119 bp

Back to Microtropis

Pi 0.0108

MarkerSeek score 59.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1119
variable_sites: 12
pi: 0.0108
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	GTTCCCTCATTTCCATCCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	57.9	58.1	1196-1207 bp	1.0	80.3
rpoB-trnC-GCA_p2	GTTCCCTCATTTCCATCCCC	TATGTTGATCAGGCGACACC	55.0	50.0	57.9	57.7	1215-1226 bp	1.0	78.4
rpoB-trnC-GCA_p3	CGTTCCCTCATTTCCATCCC	CCGGATTTGAACTGGGGAAA	55.0	50.0	58.3	58.1	1197-1208 bp	1.0	78.3
rpoB-trnC-GCA_p4	GTTCCCTCATTTCCATCCCC	AAAAGGATTTGCAGTCCCCC	55.0	50.0	57.9	58.4	1178-1189 bp	1.0	78.1
rpoB-trnC-GCA_p5	ACGTTCCCTCATTTCCATCC	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.6	58.1	1198-1209 bp	1.0	77.5