Hotspot

atpH-atpI

LSC · 14278-15512 · 1235 bp

Back to Microtropis

Pi 0.0057

MarkerSeek score 62.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1235
variable_sites: 7
pi: 0.0057
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.0	57.9	1322-1332 bp	1.0	79.5
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	CGAATCCATGGAGGGTCATC	45.0	55.0	58.0	57.8	1292-1302 bp	1.0	79.1
atpH-atpI_p3	AATAACGGAAGCGGCAGAAA	TTTTGCAACTTTAGCCGCAG	45.0	45.0	58.2	57.9	1323-1333 bp	1.0	78.3
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.2	57.8	1293-1303 bp	1.0	78.0
atpH-atpI_p5	ATAGAAGCAAGACCGACAGC	TTTTGCAACTTTAGCCGCAG	50.0	45.0	57.7	57.9	1355-1365 bp	1.0	77.3