Hotspot

rpl32-trnL-UAG

SSC · 118103-119583 · 1481 bp

Back to Micromeles

Pi 0.00378

MarkerSeek score 65.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 1481
variable_sites: 7
pi: 0.00378
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGCAGCATTGAAAGCTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	1591-1674 bp	1.0	82.8
rpl32-trnL-UAG_p2	GGGCAGCATTGAAAGCTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	1592-1675 bp	1.0	82.8
rpl32-trnL-UAG_p3	TAGGGCAGCATTGAAAGCTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	1593-1676 bp	1.0	82.5
rpl32-trnL-UAG_p4	TAGGGCAGCATTGAAAGCTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.8	58.0	1594-1677 bp	1.0	82.5
rpl32-trnL-UAG_p5	GGGCAGCATTGAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.8	57.9	1601-1684 bp	1.0	81.9