Hotspot

rps11-rpl36

LSC · 83357-83522 · 166 bp

Back to Micromeles

Pi 0.0181

MarkerSeek score 63.60

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 166
variable_sites: 4
pi: 0.0181
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	ACGTCTATTTCTACGCGAACC	AAGAGCCTCCGTTCGTAAAA	47.6	45.0	58.2	57.2	317-327 bp	1.0	64.7
rps11-rpl36_p2	CGTCTATTTCTACGCGAACCA	AAGAGCCTCCGTTCGTAAAA	47.6	45.0	58.2	57.2	316-326 bp	1.0	64.7
rps11-rpl36_p3	ACGTCTATTTCTACGCGAACC	AAATAAGAGCCTCCGTTCGT	47.6	45.0	58.2	56.9	321-331 bp	1.0	62.9
rps11-rpl36_p4	ACGTCTATTTCTACGCGAACC	AGAGCCTCCGTTCGTAAAAT	47.6	45.0	58.2	56.9	316-326 bp	1.0	62.9
rps11-rpl36_p5	CGTCTATTTCTACGCGAACCA	AGAGCCTCCGTTCGTAAAAT	47.6	45.0	58.2	56.9	315-325 bp	1.0	62.9