Hotspot

petN-psbM

LSC · 30560-31723 · 1164 bp

Back to Micromeles

Pi 0.00229

MarkerSeek score 60.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1164
variable_sites: 4
pi: 0.00229
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	AGTAAATCTCGCTTGGGCTG	AAAACAGTCAGCCAAGGTGA	50.0	45.0	58.0	57.6	1270-1325 bp	1.0	76.5
petN-psbM_p2	AGTATGGGGAAGGAGTGGAC	AAAACAGTCAGCCAAGGTGA	55.0	45.0	58.1	57.6	1213-1268 bp	1.0	75.8
petN-psbM_p3	GTATGGGGAAGGAGTGGACT	AAAACAGTCAGCCAAGGTGA	55.0	45.0	58.1	57.6	1212-1267 bp	1.0	75.8
petN-psbM_p4	TATGGGGAAGGAGTGGACTC	AAAACAGTCAGCCAAGGTGA	55.0	45.0	57.8	57.6	1211-1266 bp	1.0	75.4
petN-psbM_p5	GGGGAAGGAGTGGACTCTAG	AAAACAGTCAGCCAAGGTGA	60.0	45.0	58.2	57.6	1208-1263 bp	1.0	75.0