Hotspot

trnR-UCU-atpA

LSC · 10195-11202 · 1008 bp

Back to Micromeles

Pi 0.0518

MarkerSeek score 90.70

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 1008
variable_sites: 61
pi: 0.0518
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 90.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	TGGAATGAAAAGCGTCCATTG	AGACATTTACCGACGAAGCG	42.9	50.0	57.4	58.4	917-1205 bp	1.0	72.8
trnR-UCU-atpA_p2	GGAATGAAAAGCGTCCATTGT	AGACATTTACCGACGAAGCG	42.9	50.0	57.4	58.4	916-1204 bp	1.0	72.8
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	AGACATTTACCGACGAAGCG	47.6	50.0	57.3	58.4	856-1144 bp	1.0	72.1
trnR-UCU-atpA_p4	TGGAATGAAAAGCGTCCATT	AGACATTTACCGACGAAGCG	40.0	50.0	55.9	58.4	917-1205 bp	1.0	68.8
trnR-UCU-atpA_p5	TTGGAATGAAAAGCGTCCAT	AGACATTTACCGACGAAGCG	40.0	50.0	55.9	58.4	918-1206 bp	1.0	68.8