Hotspot

trnG-UCC-trnR-UCU

LSC · 9887-10122 · 236 bp

Back to Micromeles

Pi 0.0386

MarkerSeek score 78.70

Primer available yes

Label trnG-UCC-trnR-UCU

Feature Metadata

label_name: trnG-UCC-trnR-UCU
feature_type: igs
region: LSC
length_bp: 236
variable_sites: 9
pi: 0.0386
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-UCC-trnR-UCU_p1	AGCCTTCCAAGCTAACGATG	TGGAAATAAATTGCGTCCAATAGG	50.0	37.5	58.0	57.9	317-363 bp	1.0	52.7
trnG-UCC-trnR-UCU_p2	CCTAGCCTTCCAAGCTAACG	TGGAAATAAATTGCGTCCAATAGG	55.0	37.5	58.1	57.9	320-366 bp	1.0	52.4
trnG-UCC-trnR-UCU_p3	AGCCTTCCAAGCTAACGATG	TGGAAATAAATTGCGTCCAA	50.0	35.0	58.0	53.8	317-363 bp	1.0	51.9
trnG-UCC-trnR-UCU_p4	CCTAGCCTTCCAAGCTAACG	TGGAAATAAATTGCGTCCAA	55.0	35.0	58.1	53.8	320-366 bp	1.0	51.7
trnG-UCC-trnR-UCU_p5	AGCCTTCCAAGCTAACGATG	AGGTTTAGAAGACCTATGTCCT	50.0	40.9	58.0	55.6	276-322 bp	1.0	51.1