Hotspot

ccsA-ndhD

SSC · 113382-113572 · 191 bp

Back to Micranthes

Pi 0.066

MarkerSeek score 77.80

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 191
variable_sites: 31
pi: 0.066
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	ACCCAAGGCTTTGCTACTTC	TACCCCGCGCTCATTCTATA	50.0	50.0	58.1	58.1	1516-1631 bp	1.0	86.4
ccsA-ndhD_p2	CAAATTCCGCAATTGTGGCT	ATCGATGGCTTCACTTGCAT	45.0	45.0	57.9	58.0	656-771 bp	0.833333	77.2
ccsA-ndhD_p3	CAAATTCCGCAATTGTGGCT	TTCAACATCTCCTGGCCAAC	45.0	50.0	57.9	58.1	923-1038 bp	0.833333	76.5
ccsA-ndhD_p4	CAAATTCCGCAATTGTGGCT	TACCCCGCGCTCATTCTATA	45.0	50.0	57.9	58.1	993-1108 bp	0.833333	76.4
ccsA-ndhD_p5	ACCCAAGGCTTTGCTACTTC	ATCGATGGCTTCACTTGCAT	50.0	45.0	58.1	58.0	1179-1294 bp	0.833333	76.3