Hotspot

rpl32-trnL-UAG

SSC · 111761-112235 · 475 bp

Back to Micranthes

Pi 0.0563

MarkerSeek score 82.60

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 475
variable_sites: 69
pi: 0.0563
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGATATTGGGCAGCGTTAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	577-675 bp	1.0	91.4
rpl32-trnL-UAG_p2	GGGATATTGGGCAGCGTTAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	578-676 bp	1.0	91.4
rpl32-trnL-UAG_p3	GGGATATTGGGCAGCGTTAA	GAAGTAGCAAAGCCTTGGGT	50.0	50.0	58.0	58.1	1069-1249 bp	1.0	91.1
rpl32-trnL-UAG_p4	TTGACCCATTGAGCTTGACC	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.1	58.0	1137 bp	0.166667	45.9
rpl32-trnL-UAG_p5	TTGACCCATTGAGCTTGACC	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.1	58.0	1138 bp	0.166667	45.9