Hotspot

trnT-UGU-trnL-UAA

LSC · 45335-46283 · 949 bp

Back to Micranthes

Pi 0.0511

MarkerSeek score 83.30

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 949
variable_sites: 115
pi: 0.0511
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GTTGGACGTTCCTACAGCAT	GCCTTCAGCCTTTCGATGTA	50.0	50.0	57.9	58.0	1276-1416 bp	0.833333	83.0
trnT-UGU-trnL-UAA_p2	GTTGGACGTTCCTACAGCAT	ACCAATTTCGCCATATCCCC	50.0	50.0	57.9	58.0	1054-1235 bp	0.833333	81.7
trnT-UGU-trnL-UAA_p3	TTGGAAAAGGGGAGAAAGCC	ACCAATTTCGCCATATCCCC	50.0	50.0	58.0	58.0	1018-1136 bp	0.666667	71.6
trnT-UGU-trnL-UAA_p4	TTGGAAAAGGGGAGAAAGCC	GCCTTCAGCCTTTCGATGTA	50.0	50.0	58.0	58.0	1240-1359 bp	0.666667	71.3
trnT-UGU-trnL-UAA_p5	TTGGAAAAGGGGAGAAAGCC	TAGCGTCTACCAATTTCGCC	50.0	50.0	58.0	58.1	1026-1144 bp	0.666667	71.0