Hotspot

psbM-trnD-GUC

LSC · 29185-29629 · 445 bp

Back to Micranthes

Pi 0.0733

MarkerSeek score 84.60

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 445
variable_sites: 71
pi: 0.0733
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 84.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AAATGCAAGAATATTTACTTCCA	TTCAATTGGTCAGAGCACCG	26.1	50.0	52.7	58.5	506-941 bp	1.0	52.0
psbM-trnD-GUC_p2	AATGAACAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	44.4	50.0	52.6	58.5	527-962 bp	1.0	51.9
psbM-trnD-GUC_p3	AATGAACAGTGCAGTAGC	GTTCAATTGGTCAGAGCACC	44.4	50.0	52.6	57.0	528-963 bp	1.0	51.9
psbM-trnD-GUC_p4	AATGAACAGTGCAGTAGC	TCAATTGGTCAGAGCACCG	44.4	52.6	52.6	57.8	526-961 bp	1.0	51.9
psbM-trnD-GUC_p5	AATGAACAGTGCAGTAGC	AGTTCAATTGGTCAGAGCACC	44.4	47.6	52.6	58.5	529-964 bp	1.0	51.9