Hotspot

rpoB-trnC-GCA

LSC · 26444-27636 · 1193 bp

Back to Micranthes

Pi 0.0424

MarkerSeek score 82.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1193
variable_sites: 130
pi: 0.0424
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	ATTCCCTCATTTCCATCCCG	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.3	58.1	1214-1281 bp	1.0	86.0
rpoB-trnC-GCA_p2	ATTCCCTCATTTCCATCCCG	AAAAGGATTTGCAGTCCCCC	50.0	50.0	57.3	58.4	1196-1263 bp	1.0	83.8
rpoB-trnC-GCA_p3	ACATTCCCTCATTTCCATCCC	CCGGATTTGAACTGGGGAAA	47.6	50.0	57.7	58.1	1216-1283 bp	1.0	80.8
rpoB-trnC-GCA_p4	ATTCCCTCATTTCCATCCCG	GACACCCGGATTTGAACTGG	50.0	55.0	57.3	58.8	1219-1286 bp	1.0	80.0
rpoB-trnC-GCA_p5	TTCCCTCATTTCCATCCCGG	CCGGATTTGAACTGGGGAAA	55.0	50.0	59.4	58.1	1213-1280 bp	1.0	79.8