Hotspot

atpH-atpI

LSC · 13305-14247 · 943 bp

Back to Micranthes

Pi 0.0452

MarkerSeek score 83.60

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 943
variable_sites: 104
pi: 0.0452
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TCTATGGAGGGTCATCATTGA	45.0	42.9	58.0	55.5	869-1061 bp	1.0	64.0
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	TCTATGGAGGGTCATCATTGA	45.0	42.9	57.9	55.5	861-1053 bp	1.0	63.8
atpH-atpI_p3	AAGCGGCAGAAATCAATGGA	TCTATGGAGGGTCATCATTGA	45.0	42.9	58.2	55.5	862-1054 bp	1.0	63.0
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	TCTATGGAGGGTCATCATTGA	45.0	42.9	58.2	55.5	870-1062 bp	1.0	62.9
atpH-atpI_p5	GAAGCGGCAGAAATCAATGG	TCTATGGAGGGTCATCATTGA	50.0	42.9	57.8	55.5	863-1055 bp	1.0	62.7