Hotspot

trnL-GAG-trnS-GGA

Genome · 117095-117552 · 458 bp

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Pi 0.395

MarkerSeek score 78.00

Primer available yes

Label trnL-GAG-trnS-GGA

Feature Metadata

label_name: trnL-GAG-trnS-GGA
feature_type: igs
region: Genome
length_bp: 458
variable_sites: 181
pi: 0.395
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-GAG-trnS-GGA_p1	AAACCCCTCAAGCTTGTGTG	TTCGAATCCCTCTCTTTCCG	50.0	50.0	58.6	57.0	521-1239 bp	1.0	75.5
trnL-GAG-trnS-GGA_p2	CAAACCCCTCAAGCTTGTGT	TTCGAATCCCTCTCTTTCCG	50.0	50.0	58.6	57.0	522-1240 bp	1.0	75.5
trnL-GAG-trnS-GGA_p3	AAACCCCTCAAGCTTGTGTG	GGGTTCGAATCCCTCTCTTT	50.0	50.0	58.6	56.9	524-1242 bp	1.0	74.6
trnL-GAG-trnS-GGA_p4	CAAACCCCTCAAGCTTGTGT	GGGTTCGAATCCCTCTCTTT	50.0	50.0	58.6	56.9	525-1243 bp	1.0	74.6
trnL-GAG-trnS-GGA_p5	AAACCCCTCAAGCTTGTGTG	GGTTCGAATCCCTCTCTTTCC	50.0	52.4	58.6	58.1	523-1241 bp	1.0	74.5