Hotspot

rrn16-trnL-GAG

Genome · 116055-117009 · 955 bp

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Pi 0.143

MarkerSeek score 84.20

Primer available yes

Label rrn16-trnL-GAG

Feature Metadata

label_name: rrn16-trnL-GAG
feature_type: igs
region: Genome
length_bp: 955
variable_sites: 132
pi: 0.143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnL-GAG_p1	TAACGCTTGCATCCTCTGTC	TCCATCCCGGTTCGAGTC	50.0	61.1	58.0	58.4	1030-1385 bp	1.0	70.9
rrn16-trnL-GAG_p2	ATTCCGGATAACGCTTGCAT	TCCATCCCGGTTCGAGTC	45.0	61.1	58.0	58.4	1038-1393 bp	1.0	70.7
rrn16-trnL-GAG_p3	TAACGCTTGCATCCTCTGTC	GTTCGAGTCCGGGTAACG	50.0	61.1	58.0	57.5	1021-1376 bp	1.0	70.3
rrn16-trnL-GAG_p4	TTTAAGCCACCTACAGACGC	TCCATCCCGGTTCGAGTC	50.0	61.1	57.9	58.4	1071-1426 bp	1.0	70.2
rrn16-trnL-GAG_p5	CGCTTTACGCCCAATCATTC	TCCATCCCGGTTCGAGTC	50.0	61.1	57.9	58.4	1054-1409 bp	1.0	70.1