Hotspot

trnT-GGU-cysA

Genome · 105936-106794 · 859 bp

Back to Micractinium

Pi 0.315

MarkerSeek score 77.50

Primer available yes

Label trnT-GGU-cysA

Feature Metadata

label_name: trnT-GGU-cysA
feature_type: igs
region: Genome
length_bp: 859
variable_sites: 188
pi: 0.315
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.69
markerseek_score: 77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-cysA_p1	TAGCCCTGTTCCTTTCCTCA	GAAAACAGCTTCGTTCGTGG	50.0	50.0	58.0	58.0	1204-1459 bp	1.0	91.5
trnT-GGU-cysA_p2	TAGCCCTGTTCCTTTCCTCA	TAGCCGTTGAGCCAAAAGTT	50.0	45.0	58.0	58.0	1267-1522 bp	1.0	91.5
trnT-GGU-cysA_p3	TGACGGGGAGTTTCAGTACT	GAAAACAGCTTCGTTCGTGG	50.0	50.0	58.0	58.0	1327 bp	0.5	59.8
trnT-GGU-cysA_p4	TGACGGGGAGTTTCAGTACT	TAGCCGTTGAGCCAAAAGTT	50.0	45.0	58.0	58.0	1390 bp	0.5	59.8
trnT-GGU-cysA_p5	TGACGGGGAGTTTCAGTACT	TTAGCCGTTGAGCCAAAAGT	50.0	45.0	58.0	58.0	1391 bp	0.5	59.8