Hotspot

psbZ-trnG-UCC

Genome · 96718-97338 · 621 bp

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Pi 0.0945

MarkerSeek score 86.30

Primer available yes

Label psbZ-trnG-UCC

Feature Metadata

label_name: psbZ-trnG-UCC
feature_type: igs
region: Genome
length_bp: 621
variable_sites: 57
pi: 0.0945
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 86.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-UCC_p1	AACAGGCACACCAACAACTA	CAAGGGCACTACCACTATGC	45.0	55.0	57.6	58.3	725-732 bp	1.0	82.2
psbZ-trnG-UCC_p2	ACAGGCACACCAACAACTAA	CAAGGGCACTACCACTATGC	45.0	55.0	57.6	58.3	724-731 bp	1.0	82.2
psbZ-trnG-UCC_p3	ACAGGCACACCAACAACTAA	GCAAGGGCACTACCACTATG	45.0	55.0	57.6	58.3	725-732 bp	1.0	82.2
psbZ-trnG-UCC_p4	AACAGGCACACCAACAACTA	GCAAGGGCACTACCACTATG	45.0	55.0	57.6	58.3	726-733 bp	1.0	82.1
psbZ-trnG-UCC_p5	ACAGGCACACCAACAACTAA	GGGCACTACCACTATGCAAT	45.0	50.0	57.6	57.7	721-728 bp	1.0	82.1