Hotspot

trnI-UAU-psaB

Genome · 82913-83621 · 709 bp

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Pi 0.184
MarkerSeek score 86.70
Primer available yes
Label trnI-UAU-psaB

Feature Metadata

label_name
trnI-UAU-psaB
feature_type
igs
region
Genome
length_bp
709
variable_sites
108
pi
0.184
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.83
markerseek_score
86.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-UAU-psaB_p1 TTCAAACCCAACTGGATGCA GCGGCATTCTTAATTGCTTCA 45.0 42.9 57.9 57.9 727-771 bp 1.0 82.2
trnI-UAU-psaB_p2 TTCAAACCCAACTGGATGCA TGCGGCATTCTTAATTGCTTC 45.0 42.9 57.9 57.9 728-772 bp 1.0 82.2
trnI-UAU-psaB_p3 TTCAAACCCAACTGGATGCA TGCGGCATTCTTAATTGCTT 45.0 40.0 57.9 56.7 728-772 bp 1.0 80.8
trnI-UAU-psaB_p4 ACTCATAATGCGGCTTCGAA GCGGCATTCTTAATTGCTTCA 45.0 42.9 57.7 57.9 760-804 bp 1.0 80.3
trnI-UAU-psaB_p5 ACTCATAATGCGGCTTCGAA TGCGGCATTCTTAATTGCTTC 45.0 42.9 57.7 57.9 761-805 bp 1.0 80.3