Hotspot

psbA-ycf1

Genome · 67668-68671 · 1004 bp

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Pi 0.25

MarkerSeek score 78.20

Primer available yes

Label psbA-ycf1

Feature Metadata

label_name: psbA-ycf1
feature_type: igs
region: Genome
length_bp: 1004
variable_sites: 184
pi: 0.25
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-ycf1_p1	GGATCGCAGTTGCTTACTCA	CGATGGCGCAAAGAGTTTTT	50.0	45.0	58.0	57.9	2109-2342 bp	1.0	91.5
psbA-ycf1_p2	TGGATCGCAGTTGCTTACTC	CGATGGCGCAAAGAGTTTTT	50.0	45.0	58.0	57.9	2110-2343 bp	1.0	91.5
psbA-ycf1_p3	TTCTCTGCTATGCACGGTTC	CGATGGCGCAAAGAGTTTTT	50.0	45.0	58.0	57.9	1903-2136 bp	1.0	91.5
psbA-ycf1_p4	AACGCTCACAACTTCCCATT	CGATGGCGCAAAGAGTTTTT	45.0	45.0	58.0	57.9	1531-1764 bp	1.0	91.4
psbA-ycf1_p5	TTCTCTGCTATGCACGGTTC	ATGACCCAGAAGTGACTCGA	50.0	50.0	58.0	58.1	1559-1792 bp	1.0	91.3