Hotspot

petD-trnC-GCA

Genome · 48953-50502 · 1550 bp

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Pi 0.199

MarkerSeek score 81.90

Primer available yes

Label petD-trnC-GCA

Feature Metadata

label_name: petD-trnC-GCA
feature_type: igs
region: Genome
length_bp: 1550
variable_sites: 256
pi: 0.199
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.83
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-trnC-GCA_p1	TACAGTGGCAGCAATTTGGT	AAAGGTTTGGGGAAGAGCTG	45.0	50.0	58.0	58.0	1945 bp	0.5	59.9
petD-trnC-GCA_p2	TACAGTGGCAGCAATTTGGT	CAAAGGTTTGGGGAAGAGCT	45.0	50.0	58.0	58.0	1946 bp	0.5	59.9
petD-trnC-GCA_p3	TGGTACAGTGGCAGCAATTT	AAAGGTTTGGGGAAGAGCTG	45.0	50.0	58.0	58.0	1948 bp	0.5	59.9
petD-trnC-GCA_p4	TGGTACAGTGGCAGCAATTT	CAAAGGTTTGGGGAAGAGCT	45.0	50.0	58.0	58.0	1949 bp	0.5	59.9
petD-trnC-GCA_p5	TTGGTACAGTGGCAGCAATT	AAAGGTTTGGGGAAGAGCTG	45.0	50.0	58.0	58.0	1949 bp	0.5	59.9