Hotspot

psbD-petG

Genome · 13159-13970 · 812 bp

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Pi 0.115

MarkerSeek score 85.10

Primer available yes

Label psbD-petG

Feature Metadata

label_name: psbD-petG
feature_type: igs
region: Genome
length_bp: 812
variable_sites: 87
pi: 0.115
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 85.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-petG_p1	GAAAACGAAACGGTCACGAC	CGTCGTGGAGATCAGCTTAA	50.0	50.0	58.0	57.4	926 bp	0.5	55.5
psbD-petG_p2	CGACGAAGCCAGTCATCTAC	CGTCGTGGAGATCAGCTTAA	55.0	50.0	58.2	57.4	910 bp	0.5	54.1
psbD-petG_p3	ACCAGCCACGTTTTTCTTGA	CGTCGTGGAGATCAGCTTAA	45.0	50.0	58.2	57.4	882 bp	0.5	53.6
psbD-petG_p4	TGAAAACGAAACGGTCACGA	CGTCGTGGAGATCAGCTTAA	45.0	50.0	58.4	57.4	927 bp	0.5	52.6
psbD-petG_p5	CCAGCCACGTTTTTCTTGAG	CGTCGTGGAGATCAGCTTAA	50.0	50.0	57.6	57.4	881 bp	0.5	52.3