Hotspot

rps2-trnT-UGU

Genome · 6974-7356 · 383 bp

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Pi 0.12

MarkerSeek score 84.00

Primer available yes

Label rps2-trnT-UGU

Feature Metadata

label_name: rps2-trnT-UGU
feature_type: igs
region: Genome
length_bp: 383
variable_sites: 42
pi: 0.12
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-trnT-UGU_p1	GCGGATTCCATTCACGAGTA	TTCGACTCCGATAGAAGGCT	50.0	50.0	57.8	57.9	467-482 bp	1.0	88.9
rps2-trnT-UGU_p2	GCGGATTCCATTCACGAGTA	GTTCGACTCCGATAGAAGGC	50.0	55.0	57.8	57.9	468-483 bp	1.0	88.3
rps2-trnT-UGU_p3	ATGCCCAAAATGCATTCCAC	TTCGACTCCGATAGAAGGCT	45.0	50.0	57.6	57.9	445-460 bp	1.0	87.7
rps2-trnT-UGU_p4	ATGCCCAAAATGCATTCCAC	GTTCGACTCCGATAGAAGGC	45.0	55.0	57.6	57.9	446-461 bp	1.0	87.2
rps2-trnT-UGU_p5	GCCCAAAATGCATTCCACTT	TTCGACTCCGATAGAAGGCT	45.0	50.0	57.5	57.9	443-458 bp	1.0	87.2