Hotspot

petD

LSC · 72277-73519 · 1243 bp

Back to Metagentiana

Pi 0.00565

MarkerSeek score 47.80

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1243
variable_sites: 7
pi: 0.00565
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	TATCGTCCGACGGTTACTGA	TGAAGCCTCGCGGAATTTAA	50.0	45.0	58.0	57.9	2481-2499 bp	1.0	79.5
petD_p2	ACTATCGTCCGACGGTTACT	TGAAGCCTCGCGGAATTTAA	50.0	45.0	58.0	57.9	2483-2501 bp	1.0	79.5
petD_p3	ATTCCTTACCTCGGGACCAA	TGAAGCCTCGCGGAATTTAA	50.0	45.0	58.0	57.9	2246-2264 bp	1.0	79.4
petD_p4	TCCTTACCTCGGGACCAAAT	TGAAGCCTCGCGGAATTTAA	50.0	45.0	58.0	57.9	2244-2262 bp	1.0	79.4
petD_p5	GGTGTGGTTCTCGCTGTATT	TGAAGCCTCGCGGAATTTAA	50.0	45.0	57.9	57.9	2292-2310 bp	1.0	79.0