Hotspot

ccsA

SSC · 109158-110135 · 978 bp

Back to Metagentiana

Pi 0.00511

MarkerSeek score 47.00

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 978
variable_sites: 5
pi: 0.00511
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	AAGTTGAACACAAGCCGCTA	CTTTTCCATGGCTTCGCTTG	45.0	50.0	58.0	58.0	1720-1726 bp	1.0	80.6
ccsA_p2	TAAGTTGAACACAAGCCGCT	CTTTTCCATGGCTTCGCTTG	45.0	50.0	58.0	58.0	1721-1727 bp	1.0	80.6
ccsA_p3	AAGTTGAACACAAGCCGCTA	ATGAGTGGCTTTGTTGCTGA	45.0	45.0	58.0	58.0	1689-1695 bp	1.0	80.5
ccsA_p4	TAAGTTGAACACAAGCCGCT	ATGAGTGGCTTTGTTGCTGA	45.0	45.0	58.0	58.0	1690-1696 bp	1.0	80.5
ccsA_p5	AAGTTGAACACAAGCCGCTA	TGGTGCTGCACTCTTTTTCT	45.0	45.0	58.0	57.9	1837-1843 bp	1.0	80.2