Hotspot

trnL-UAG-ccsA

SSC · 109072-109157 · 86 bp

Back to Metagentiana

Pi 0.0349
MarkerSeek score 65.40
Primer available yes
Label trnL-UAG-ccsA

Feature Metadata

label_name
trnL-UAG-ccsA
feature_type
igs
region
SSC
length_bp
86
variable_sites
3
pi
0.0349
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-ccsA_p1 AAGTTGAACACAAGCCGCTA AGGGTTGTTAAACGAATTAGA 45.0 33.3 58.0 52.8 265 bp 1.0 40.9
trnL-UAG-ccsA_p2 TAAGTTGAACACAAGCCGCT AGGGTTGTTAAACGAATTAGA 45.0 33.3 58.0 52.8 266 bp 1.0 40.9
trnL-UAG-ccsA_p3 ACAAGCCGCTATGGTGAAAT AGGGTTGTTAAACGAATTAGA 45.0 33.3 57.9 52.8 256 bp 1.0 40.9
trnL-UAG-ccsA_p4 AAGTTGAACACAAGCCGCTA AAGGGTTGTTAAACGAATTAGA 45.0 31.8 58.0 53.7 266 bp 1.0 40.9
trnL-UAG-ccsA_p5 AAGTTGAACACAAGCCGCTA AGGGTTGTTAAACGAATTAGAA 45.0 31.8 58.0 53.7 265 bp 1.0 40.9