Hotspot

rpl32-trnL-UAG

SSC · 108112-108991 · 880 bp

Back to Metagentiana

Pi 0.0148
MarkerSeek score 60.70
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
880
variable_sites
13
pi
0.0148
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGGGGTATTGGACGGCTTTA AAAGGATGACATGCCGCTAC 50.0 50.0 58.4 58.3 1072-1084 bp 1.0 75.5
rpl32-trnL-UAG_p2 GGGGTATTGGACGGCTTTAA AAAGGATGACATGCCGCTAC 50.0 50.0 57.6 58.3 1071-1083 bp 1.0 75.4
rpl32-trnL-UAG_p3 AGGGGTATTGGACGGCTTTA TTAAAGGATGACATGCCGCT 50.0 45.0 58.4 57.6 1074-1086 bp 1.0 74.8
rpl32-trnL-UAG_p4 GGGGTATTGGACGGCTTTAA TTAAAGGATGACATGCCGCT 50.0 45.0 57.6 57.6 1073-1085 bp 1.0 74.7
rpl32-trnL-UAG_p5 AGGGGTATTGGACGGCTTTA TGCTTCCTAAGAGCAGTGTG 50.0 50.0 58.4 57.5 1024-1036 bp 1.0 74.6