Hotspot

petD-rpoA

LSC · 73520-73697 · 178 bp

Back to Metagentiana

Pi 0.0169

MarkerSeek score 62.80

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 178
variable_sites: 3
pi: 0.0169
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	TTGGTTGGGTATTGGTGCAA	ACAGGCACGAAGATCTTATGA	45.0	42.9	57.9	56.8	343 bp	1.0	62.9
petD-rpoA_p2	TTTGATTGGTACCGGGGTTG	ACAGGCACGAAGATCTTATGA	50.0	42.9	58.1	56.8	367 bp	1.0	62.8
petD-rpoA_p3	TTTGGTTGGGTATTGGTGCA	ACAGGCACGAAGATCTTATGA	45.0	42.9	57.9	56.8	344 bp	1.0	62.8
petD-rpoA_p4	TTTGATTGGTACCGGGGTTG	ACAATAACAGGCACGAAGATCT	50.0	40.9	58.1	57.8	373 bp	1.0	62.5
petD-rpoA_p5	TTGGTTGGGTATTGGTGCAA	ACAATAACAGGCACGAAGATCT	45.0	40.9	57.9	57.8	349 bp	1.0	62.5