Hotspot

trnP-UGG-psaJ

LSC · 62467-62843 · 377 bp

Back to Metagentiana

Pi 0.0133
MarkerSeek score 61.70
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
377
variable_sites
5
pi
0.0133
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 ACCCGTGACATTTTGTACCC GCGTACTTAGTACTGGTGCC 50.0 55.0 58.1 58.1 481 bp 1.0 79.4
trnP-UGG-psaJ_p2 CCCGTGACATTTTGTACCCA GCGTACTTAGTACTGGTGCC 50.0 55.0 58.1 58.1 480 bp 1.0 79.4
trnP-UGG-psaJ_p3 ACAGGATTTGAACCCGTGAC GCGTACTTAGTACTGGTGCC 50.0 55.0 58.1 58.1 492 bp 1.0 79.3
trnP-UGG-psaJ_p4 CAGGATTTGAACCCGTGACA GCGTACTTAGTACTGGTGCC 50.0 55.0 58.1 58.1 491 bp 1.0 79.3
trnP-UGG-psaJ_p5 TGACAGGATTTGAACCCGTG GCGTACTTAGTACTGGTGCC 50.0 55.0 58.1 58.1 494 bp 1.0 79.3