Hotspot

psbE-petL

LSC · 60965-61644 · 680 bp

Back to Metagentiana

Pi 0.0118

MarkerSeek score 56.40

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 680
variable_sites: 8
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	CAACCCGCAATGAATAGGGA	TCGTTAAAGCCGCGAGTAAA	50.0	45.0	57.9	57.9	833-848 bp	1.0	79.9
psbE-petL_p2	ACGAATAACCAACCCGCAAT	TCGTTAAAGCCGCGAGTAAA	45.0	45.0	58.2	57.9	842-857 bp	1.0	79.0
psbE-petL_p3	TGCTGACGAATAACCAACCC	TCGTTAAAGCCGCGAGTAAA	50.0	45.0	58.2	57.9	847-862 bp	1.0	78.9
psbE-petL_p4	GTTCTCCTGTGCTTCCAGAC	TCGTTAAAGCCGCGAGTAAA	55.0	45.0	58.2	57.9	751-766 bp	1.0	78.9
psbE-petL_p5	CGAATAACCAACCCGCAATG	TCGTTAAAGCCGCGAGTAAA	50.0	45.0	57.8	57.9	841-856 bp	1.0	78.8