Hotspot

rps14-psaB

LSC · 34832-34965 · 134 bp

Back to Metagentiana

Pi 0.0224

MarkerSeek score 63.80

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 134
variable_sites: 3
pi: 0.0224
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	TGTCTCTTTTTCTCCCGCTG	TTGCTTCTACATCGGGCAAA	50.0	45.0	57.8	57.8	204 bp	1.0	78.3
rps14-psaB_p2	CTCCCGCTGAATCAAACCTT	TTGCTTCTACATCGGGCAAA	50.0	45.0	58.2	57.8	193 bp	1.0	78.3
rps14-psaB_p3	TGTCTCTTTTTCTCCCGCTG	AGTAGCCCTTTCCATTGTGC	50.0	50.0	57.8	58.2	290 bp	1.0	78.2
rps14-psaB_p4	CTCCCGCTGAATCAAACCTT	AGTAGCCCTTTCCATTGTGC	50.0	50.0	58.2	58.2	279 bp	1.0	78.2
rps14-psaB_p5	TTCTCCCGCTGAATCAAACC	AGTAGCCCTTTCCATTGTGC	50.0	50.0	58.2	58.2	281 bp	1.0	78.1