Hotspot

trnK-UUU-trnQ-UUG

LSC · 4247-5363 · 1117 bp

Back to Metagentiana

Pi 0.0082
MarkerSeek score 57.10
Primer available yes
Label trnK-UUU-trnQ-UUG

Feature Metadata

label_name
trnK-UUU-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1117
variable_sites
9
pi
0.0082
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-trnQ-UUG_p1 CGAATCCCTTGCTTCATCCA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1216-1223 bp 1.0 81.0
trnK-UUU-trnQ-UUG_p2 ACGAATCCCTTGCTTCATCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1217-1224 bp 1.0 80.9
trnK-UUU-trnQ-UUG_p3 ATGGACGAATCCCTTGCTTC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1221-1228 bp 1.0 80.9
trnK-UUU-trnQ-UUG_p4 GGACGAATCCCTTGCTTCAT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1219-1226 bp 1.0 80.9
trnK-UUU-trnQ-UUG_p5 CGATGGTATGGACGAATCCC GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.9 58.1 1228-1235 bp 1.0 80.6