Hotspot

trnH-GUG-psbA

LSC · 74-395 · 322 bp

Back to Metagentiana

Pi 0.035

MarkerSeek score 63.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 322
variable_sites: 11
pi: 0.035
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.9	58.3	447-448 bp	1.0	79.6
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	CCGTGCTAACCTTGGTATGG	50.0	55.0	58.2	58.3	448-449 bp	1.0	79.4
trnH-GUG-psbA_p3	CTGCCTTAATCCACTTGGCT	ACCGTGCTAACCTTGGTATG	50.0	50.0	57.9	57.6	448-449 bp	1.0	79.1
trnH-GUG-psbA_p4	ACTGCCTTAATCCACTTGGC	ACCGTGCTAACCTTGGTATG	50.0	50.0	58.2	57.6	449-450 bp	1.0	78.9
trnH-GUG-psbA_p5	AATCCACTTGGCTACATCCG	CCGTGCTAACCTTGGTATGG	50.0	55.0	57.7	58.3	440-441 bp	1.0	78.1