Hotspot

petA-psbJ

LSC · 111362-112500 · 1139 bp

Back to Mesoamerantha

Pi 0.0186

MarkerSeek score 57.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1139
variable_sites: 21
pi: 0.0186
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GCAATCCCAATGTAGGAGGG	TCTGGGTTGGGTTCATCTCT	55.0	50.0	58.0	58.0	1328-1336 bp	1.0	81.4
petA-psbJ_p2	GCAATCCCAATGTAGGAGGG	TGGGTTGGGTTCATCTCTCT	55.0	50.0	58.0	58.0	1326-1334 bp	1.0	81.4
petA-psbJ_p3	CAGGGCCAGAACTTCTTGTT	TCTGGGTTGGGTTCATCTCT	50.0	50.0	58.0	58.0	1388-1396 bp	1.0	81.3
petA-psbJ_p4	CAGGGCCAGAACTTCTTGTT	TGGGTTGGGTTCATCTCTCT	50.0	50.0	58.0	58.0	1386-1394 bp	1.0	81.3
petA-psbJ_p5	GCAATCCCAATGTAGGAGGG	GGACTAGCTGTACCTACCGT	55.0	55.0	58.0	58.0	1757-1765 bp	1.0	81.0