Hotspot

accD-psaI

LSC · 106766-107640 · 875 bp

Back to Mesoamerantha

Pi 0.022

MarkerSeek score 60.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 875
variable_sites: 19
pi: 0.022
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GGTTTCTTTCCCTTGAATCA	CCATTGCAATTGCCGGAAAT	40.0	45.0	53.4	58.0	972-981 bp	1.0	45.3
accD-psaI_p2	GGTTTCTTTCCCTTGAATCA	AAGAAGCCATTGCAATTGCC	40.0	45.0	53.4	57.9	978-987 bp	1.0	44.6
accD-psaI_p3	GGTTTCTTTCCCTTGAATCAA	CCATTGCAATTGCCGGAAAT	38.1	45.0	54.3	58.0	972-981 bp	1.0	44.5
accD-psaI_p4	GGTTTCTTTCCCTTGAATCAA	AAGAAGCCATTGCAATTGCC	38.1	45.0	54.3	57.9	978-987 bp	1.0	43.8
accD-psaI_p5	GGTTTCTTTCCCTTGAATCA	ATTGCCGGAAATACTAGGGC	40.0	50.0	53.4	57.7	964-973 bp	1.0	43.3