Hotspot

trnV-UAC-trnM-CAU

LSC · 100002-100178 · 177 bp

Back to Mesoamerantha

Pi 0.0299
MarkerSeek score 69.10
Primer available yes
Label trnV-UAC-trnM-CAU

Feature Metadata

label_name
trnV-UAC-trnM-CAU
feature_type
igs
region
LSC
length_bp
177
variable_sites
5
pi
0.0299
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.94
markerseek_score
69.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-UAC-trnM-CAU_p1 CTCCCTTGAAAAGCATTGGC TTGAACCAATGACTCCTGCC 50.0 50.0 57.6 58.1 288-298 bp 1.0 80.4
trnV-UAC-trnM-CAU_p2 CTCCCTTGAAAAGCATTGGC CCATTGCATTGACTCCGGTA 50.0 50.0 57.6 58.0 335-345 bp 1.0 80.2
trnV-UAC-trnM-CAU_p3 CTCCCTTGAAAAGCATTGGC TACCATTGCATTGACTCCGG 50.0 50.0 57.6 58.0 337-347 bp 1.0 80.2
trnV-UAC-trnM-CAU_p4 GCTCCCTTGAAAAGCATTGG CCATTGCATTGACTCCGGTA 50.0 50.0 57.6 58.0 336-346 bp 1.0 80.2
trnV-UAC-trnM-CAU_p5 GCTCCCTTGAAAAGCATTGG TACCATTGCATTGACTCCGG 50.0 50.0 57.6 58.0 338-348 bp 1.0 80.2