Hotspot

trnF-GAA-ndhJ

LSC · 95810-96569 · 760 bp

Back to Mesoamerantha

Pi 0.0239

MarkerSeek score 65.60

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 760
variable_sites: 18
pi: 0.0239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CCTCGTGTCACCAGTTCAAA	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.1	57.9	870-881 bp	1.0	80.8
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.1	58.2	860-871 bp	1.0	80.3
trnF-GAA-ndhJ_p3	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	913-924 bp	1.0	79.5
trnF-GAA-ndhJ_p4	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	912-923 bp	1.0	79.5
trnF-GAA-ndhJ_p5	ATGGTCGGGATAGCTCAGTT	ATAGGCTGGCCCTTACGTAA	50.0	50.0	58.2	58.2	902-913 bp	1.0	78.9