Hotspot

atpF-atpH

LSC · 59616-59794 · 179 bp

Back to Mesoamerantha

Pi 0.0335

MarkerSeek score 71.80

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 179
variable_sites: 6
pi: 0.0335
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	GTGGCGTGAGAAAACGAAAG	GTTGTGGCATTAGCGCTTTT	50.0	45.0	58.0	57.9	260-529 bp	1.0	80.6
atpF-atpH_p2	TGGCGTGAGAAAACGAAAGA	GTTGTGGCATTAGCGCTTTT	45.0	45.0	58.1	57.9	259-528 bp	1.0	80.2
atpF-atpH_p3	AGTGGCGTGAGAAAACGAAA	GTTGTGGCATTAGCGCTTTT	45.0	45.0	58.3	57.9	261-530 bp	1.0	78.0
atpF-atpH_p4	GTGGCGTGAGAAAACGAAAG	ATTTACGGACTGGTTGTGGC	50.0	50.0	58.0	58.5	272-541 bp	1.0	77.3
atpF-atpH_p5	TGGCGTGAGAAAACGAAAGA	ATTTACGGACTGGTTGTGGC	45.0	50.0	58.1	58.5	271-540 bp	1.0	77.0