Hotspot

rps16-trnQ-UUG

LSC · 51396-52915 · 1520 bp

Back to Mesoamerantha

Pi 0.0195

MarkerSeek score 65.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1520
variable_sites: 29
pi: 0.0195
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CAGCCGATCATGTCCTTCAA	GAAATCAAAATGGGGCGTGG	50.0	50.0	58.0	58.0	1634-1669 bp	1.0	82.8
rps16-trnQ-UUG_p2	GCCGATCATGTCCTTCAAGT	GAAATCAAAATGGGGCGTGG	50.0	50.0	58.0	58.0	1632-1667 bp	1.0	82.8
rps16-trnQ-UUG_p3	AATCCACAGCCGATCATGTC	GAAATCAAAATGGGGCGTGG	50.0	50.0	58.0	58.0	1640-1675 bp	1.0	82.7
rps16-trnQ-UUG_p4	CAGCCGATCATGTCCTTCAA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1576-1611 bp	1.0	82.4
rps16-trnQ-UUG_p5	GCCGATCATGTCCTTCAAGT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	1574-1609 bp	1.0	82.4