Hotspot

psaC-ndhE

SSC · 7536-7925 · 390 bp

Back to Mesoamerantha

Pi 0.0308

MarkerSeek score 69.60

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 390
variable_sites: 12
pi: 0.0308
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	TTGCTTGGCTTTACATCCGT	TTATAGCCATTGCAGCTGCT	45.0	45.0	58.1	57.6	614-627 bp	1.0	78.0
psaC-ndhE_p2	ACATCCGTCCCAAGGTATCA	TTATAGCCATTGCAGCTGCT	50.0	45.0	58.1	57.6	602-615 bp	1.0	77.7
psaC-ndhE_p3	CCAATACATCTGTGGGGCAA	TTATAGCCATTGCAGCTGCT	50.0	45.0	57.9	57.6	579-592 bp	1.0	77.7
psaC-ndhE_p4	TTGCTTGGCTTTACATCCGT	TATAGCCATTGCAGCTGCTG	45.0	50.0	58.1	58.4	613-626 bp	1.0	77.6
psaC-ndhE_p5	TTGCTTGGCTTTACATCCGT	GTTATAGCCATTGCAGCTGC	45.0	50.0	58.1	57.6	615-628 bp	1.0	77.5