Hotspot

ndhD-psaC

SSC · 7185-7289 · 105 bp

Back to Mesoamerantha

Pi 0.0952

MarkerSeek score 72.20

Primer available yes

Label ndhD-psaC

Feature Metadata

label_name: ndhD-psaC
feature_type: igs
region: SSC
length_bp: 105
variable_sites: 10
pi: 0.0952
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-psaC_p1	AATTGGCCAAGCTGCAAAAG	CTGAAACAACTCGCAGCATG	45.0	50.0	58.1	58.0	488 bp	1.0	81.4
ndhD-psaC_p2	TTTGACCGCTGTACATTGCT	CTGAAACAACTCGCAGCATG	45.0	50.0	58.1	58.0	543 bp	1.0	81.3
ndhD-psaC_p3	TGTAGCCGAGTAGAGACGTT	CTGAAACAACTCGCAGCATG	50.0	50.0	57.9	58.0	662 bp	1.0	81.1
ndhD-psaC_p4	TTGTAGCCGAGTAGAGACGT	CTGAAACAACTCGCAGCATG	50.0	50.0	57.9	58.0	663 bp	1.0	81.1
ndhD-psaC_p5	ACAACCGCGAATTTCGAGTA	CTGAAACAACTCGCAGCATG	45.0	50.0	57.9	58.0	507 bp	0.5	59.1