Hotspot

ccsA-ndhD

SSC · 5390-5654 · 265 bp

Back to Mesoamerantha

Pi 0.0514

MarkerSeek score 79.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 265
variable_sites: 13
pi: 0.0514
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 79.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	GGAAAGGTACGAATTCGGCA	CCCGATTTCGTTCTCTCGTT	50.0	50.0	58.3	58.0	438-450 bp	1.0	82.3
ccsA-ndhD_p2	TGGAAAGGTACGAATTCGGC	CCCGATTTCGTTCTCTCGTT	50.0	50.0	58.3	58.0	439-451 bp	1.0	82.3
ccsA-ndhD_p3	AGGTACGAATTCGGCACTTG	CCCGATTTCGTTCTCTCGTT	50.0	50.0	58.3	58.0	434-446 bp	1.0	82.3
ccsA-ndhD_p4	GGAAAGGTACGAATTCGGCA	CAGTTGACAAGGTACACGCT	50.0	50.0	58.3	58.1	416-428 bp	1.0	81.5
ccsA-ndhD_p5	TGGAAAGGTACGAATTCGGC	CAGTTGACAAGGTACACGCT	50.0	50.0	58.3	58.1	417-429 bp	1.0	81.5