Hotspot

petD

LSC · 77913-79164 · 1252 bp

Back to Mesembryanthemum

Pi 0.013

MarkerSeek score 58.10

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1252
variable_sites: 16
pi: 0.013
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ACATAACACCACTAGTTGAAGGT	TTCCCTAGATACGCATGTCG	39.1	50.0	57.4	56.9	1319-1342 bp	1.0	45.2
petD_p2	ACATAACACCACTAGTTGAAGGT	GCGACTATTCCCTAGATACGC	39.1	52.4	57.4	57.9	1326-1349 bp	1.0	44.8
petD_p3	ACATAACACCACTAGTTGAAGGT	CGCATGTCGTGTTATTTCACA	39.1	42.9	57.4	57.6	1308-1331 bp	1.0	42.4
petD_p4	CACTAGTTGAAGGTAATTCTCCGA	TTCCCTAGATACGCATGTCG	41.7	50.0	58.0	56.9	1310-1333 bp	1.0	42.4
petD_p5	ACCACTAGTTGAAGGTAATTCTCC	TTCCCTAGATACGCATGTCG	41.7	50.0	57.9	56.9	1312-1335 bp	1.0	42.4