Hotspot

trnL-UAG-ccsA

SSC · 114708-114838 · 131 bp

Back to Mesembryanthemum

Pi 0.0407

MarkerSeek score 77.20

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 131
variable_sites: 5
pi: 0.0407
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	CTCTTAGGAAGCAGTGCGAG	CGGAAACCGTTGAAAAGGAA	55.0	45.0	58.1	56.9	231-239 bp	1.0	74.7
trnL-UAG-ccsA_p2	CTTAGGAAGCAGTGCGAGAG	CGGAAACCGTTGAAAAGGAA	55.0	45.0	58.1	56.9	229-237 bp	1.0	74.7
trnL-UAG-ccsA_p3	AAACAAGCCGCTATGGTGAA	CGGAAACCGTTGAAAAGGAA	45.0	45.0	58.1	56.9	267-275 bp	1.0	74.0
trnL-UAG-ccsA_p4	AACAAGCCGCTATGGTGAAA	CGGAAACCGTTGAAAAGGAA	45.0	45.0	58.1	56.9	266-274 bp	1.0	74.0
trnL-UAG-ccsA_p5	TTCAAACAAGCCGCTATGGT	CGGAAACCGTTGAAAAGGAA	45.0	45.0	58.1	56.9	270-278 bp	1.0	73.9