Hotspot

ndhF-rpl32

SSC · 112732-113621 · 890 bp

Back to Mesembryanthemum

Pi 0.0188

MarkerSeek score 64.20

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 890
variable_sites: 16
pi: 0.0188
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	TGTTGCTGTCGGAAAAAGGA	ACGCTTTTTAGAAAGAGAAGTACG	45.0	37.5	58.0	57.6	1006-1031 bp	1.0	48.4
ndhF-rpl32_p2	TGTTGCTGTCGGAAAAAGGA	CGCTTTTTAGAAAGAGAAGTACGT	45.0	37.5	58.0	57.6	1005-1030 bp	1.0	48.4
ndhF-rpl32_p3	CGGAAAAAGGAGAAGTCCCA	ACGCTTTTTAGAAAGAGAAGTACG	50.0	37.5	57.4	57.6	997-1022 bp	1.0	44.4
ndhF-rpl32_p4	CGGAAAAAGGAGAAGTCCCA	CGCTTTTTAGAAAGAGAAGTACGT	50.0	37.5	57.4	57.6	996-1021 bp	1.0	44.4
ndhF-rpl32_p5	TGTTGCTGTCGGAAAAAGGA	CGCTTTTTAGAAAGAGAAGTACG	45.0	39.1	58.0	56.0	1005-1030 bp	1.0	44.4