Hotspot

rrn4.5-rrn5

IRb · 107368-107643 · 276 bp

Back to Mesembryanthemum

Pi 0.0485

MarkerSeek score 83.00

Primer available yes

Label rrn4.5-rrn5

Feature Metadata

label_name: rrn4.5-rrn5
feature_type: igs
region: IRb
length_bp: 276
variable_sites: 13
pi: 0.0485
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn4.5-rrn5_p1	CAGCTGAGGCATCCTAACAG	AGTATCGTCACCGCAGTAGA	55.0	50.0	58.0	58.0	385-393 bp	1.0	83.7
rrn4.5-rrn5_p2	CAGCTGAGGCATCCTAACAG	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.0	58.0	383-391 bp	1.0	83.7
rrn4.5-rrn5_p3	GCTGAGGCATCCTAACAGAC	TATCGTCACCGCAGTAGAGT	55.0	50.0	58.1	58.0	381-389 bp	1.0	83.7
rrn4.5-rrn5_p4	GTCAAGTGGAAGTGCAGTGA	AGTATCGTCACCGCAGTAGA	50.0	50.0	58.0	58.0	411-419 bp	1.0	83.4
rrn4.5-rrn5_p5	GTCAAGTGGAAGTGCAGTGA	TATCGTCACCGCAGTAGAGT	50.0	50.0	58.0	58.0	409-417 bp	1.0	83.4