Hotspot

psbT-psbN

LSC · 75648-75707 · 60 bp

Back to Mesembryanthemum

Pi 0.0667

MarkerSeek score 83.30

Primer available yes

Label psbT-psbN

Feature Metadata

label_name: psbT-psbN
feature_type: igs
region: LSC
length_bp: 60
variable_sites: 4
pi: 0.0667
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 83.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbT-psbN_p1	TCGAATCTATGGAAGCATTGGT	ATCCTTTCGAAGAACACGGG	40.9	50.0	57.6	57.9	202 bp	1.0	61.4
psbT-psbN_p2	TCGAATCTATGGAAGCATTGGT	GCAGCCCTCTCAACAACTAA	40.9	50.0	57.6	57.8	225 bp	1.0	60.6
psbT-psbN_p3	TCGAATCTATGGAAGCATTGGT	TTCGAAGAACACGGGGACTA	40.9	50.0	57.6	58.4	197 bp	1.0	59.2
psbT-psbN_p4	CGAATCTATGGAAGCATTGGT	ATCCTTTCGAAGAACACGGG	42.9	50.0	56.1	57.9	201 bp	1.0	57.7
psbT-psbN_p5	CGAATCTATGGAAGCATTGGT	GCAGCCCTCTCAACAACTAA	42.9	50.0	56.1	57.8	224 bp	1.0	56.8