Hotspot

trnL-UAA-trnF-GAA

LSC · 48950-49284 · 335 bp

Back to Mesembryanthemum

Pi 0.00896

MarkerSeek score 45.90

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 335
variable_sites: 3
pi: 0.00896
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TCTACCAGCTGAGCTATCCC	50.0	55.0	57.5	58.0	389-390 bp	1.0	76.8
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	425-426 bp	1.0	76.4
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	440-441 bp	1.0	76.0
trnL-UAA-trnF-GAA_p4	AATCGTGAGGGTTCAAGTCC	TCAGTCCTCTGCTCTACCAG	50.0	55.0	57.5	57.9	401-402 bp	1.0	75.7
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TGTGCCAGGAACCAGATTTG	50.0	50.0	57.5	58.4	441-442 bp	1.0	73.7