Hotspot

atpH-atpI

LSC · 13638-14318 · 681 bp

Back to Mesembryanthemum

Pi 0.0118

MarkerSeek score 65.80

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 681
variable_sites: 8
pi: 0.0118
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AACAGAAGCGGCAGAAATCA	TTTGCAACTTTAGCTGCAGC	45.0	45.0	58.1	57.9	763-765 bp	1.0	79.1
atpH-atpI_p2	TACCTTGACCAACTCCAGGT	TTTGCAACTTTAGCTGCAGC	50.0	45.0	57.9	57.9	821-823 bp	1.0	79.0
atpH-atpI_p3	AACAGAAGCGGCAGAAATCA	CGAATCCATGGAGGGTCATC	45.0	55.0	58.1	57.8	734-736 bp	1.0	78.9
atpH-atpI_p4	AGCCAATCCAGCAGCAATAA	TTTGCAACTTTAGCTGCAGC	45.0	45.0	57.9	57.9	781-783 bp	1.0	78.7
atpH-atpI_p5	TACCTTGACCAACTCCAGGT	CGAATCCATGGAGGGTCATC	50.0	55.0	57.9	57.8	792-794 bp	1.0	78.7